The Comprehensive Guide to Germicidal UV-C

The following is intended as a valuable introduction to ultraviolet light for beginners, enthusiasts and industry partners alike


Ultraviolet light is that portion of the electromagnetic spectrum that lies beyond the purple edge of the visible spectrum and has wavelengths between 100 and 400 nm. The UV spectrum is further divided into ranges as follows: Range Name & Wavelength Range (nm)

UVA 315 - 400nm
UVB 280 -315nm
UVC 200- 280nm
Vacuum UV 100 -200nm

The UVA range causes sun tanning in the human skin. The UVB range causes sun burning. The UVC range is absorbed by DNA and thus can cause cancer and mutations. This is also the range that is most effective in inactivating bacteria and viruses. The Vacuum UV range is absorbed strongly by water and air and thus can only be transmitted in a vacuum.

Ultraviolet photons are particularly energetic and when absorbed in molecules can cause bonds to be broken (photochemistry)

What is a "photon"?

Ultraviolet light (along with all others forms of electromagnetic radiation) comes in discrete energy packets called "photons". The energy of a photon is given by: U = hn = hc/l

where h is the Planck constant (6.626755 x 10-34 J s), c is the speed of light (2.997925 x 108 m s-1), n is the frequency (Hz) of the light, is the wave number (cm-1 or m-1) of the light and l is the wavelength (nm or m). Usually photochemical "events" involve absorption of only one photon per molecule.

Why is UV important for the disinfection of surfaces, water and air?

The portion of the UV spectrum (the germicidal region) that is important for the disinfection of water and air is the range that is absorbed by DNA (RNA in some viruses). This germicidal range is approximately 200-300 nm, with a peak germicidal effectiveness at about 260 nm. The mechanism involves absorption of a UV photon by pyrimidine bases (principally thymine) where two pyrimidine bases are next to each other on the DNA chain. The photochemistry involves formation of a dimer that links the two bases together. This causes a disruption in the DNA chain, such that when the cell undergoes mitosis (cell division), the replication of DNA is inhibited

What are the major commercial applications of UV?

UV has many commercial applications in society. The major ones are: UV disinfection of water, air and surfaces, UV curing of inks and coatings, UV disinfection of foods, UV-based Advanced Oxidation destruction of pollutants in water and air.


UV-C has been an evolving technology over the past 50 years and has become the leading technology in low cost, efficient and effective disinfection in health care settings and beyond. Microorganisms, viruses, fungus and bacteria can not develop resistance to UV-C due to the photo-chemical sheering of DNA and RNA in the pathogenic genome.




UV disinfection requires an excessive amount of energy.

UV lamps have low electrical efficiencies and thus are not "energy-conscious".

The energy costs alone make UV disinfection significantly more expensive (O&M) than chemical disinfection.

Environment / Safety

The mercury contained inside the UV lamp presents a hazard to end-users (or surface waters).

Mercury contained inside the UV lamp presents an environmental hazard after disposal of used lamps.

Lamps break frequently and release mercury to the environment.

UV systems present a safety risk to plant staff because of the potential of exposure to UV-C radiation.

The visible emissions from UV lamps will cause algal growth.

UV creates DBPs that have yet to be discovered.


Lamps are always burning out and require frequent replacement.

Between lamp replacement and lamp cleaning, operations staff will spend a significant amount of time maintaining a UV disinfection system.

Lamp ballasts are unreliable and require frequent maintenance/replacement.


UV disinfection cannot be cost effectively used for 4-log virus inactivation.

Since drinking water plants have to add chlorine/chloramines for virus inactivation and residual, adding a UV system does not make financial sense unless required for Cryptosporidium inactivation.

Microorganisms can "re-activate" following UV disinfection.

Regulators won’t approve UV disinfection without significant validation and pilot testing.




The reduction of the environmental atmospheric pollution can be achieved by a natural reaction, called photocatalysis. This is realized by a photocatalyst (usually titanium dioxide) which oxidizes, and then degrades, the pollutants. The titanium dioxide is activated by solar ultraviolet radiation (UV) or by an artificial source, such as the high emission UV-C lamps placed inside a device. Nano-structured titanium dioxide (TiO2) has a large exchange surface and thus it is an excellent photocatalyst capable of degrading organic and inorganic pollutants such as VOCs, VOC (volatile organic compounds) and NOx (nitrogen oxide) produced by human activity.

Thanks to its receptive characteristics due to the fragmentation in nano particles, it has the property to oxidize, or to decompose the cells of bacteria and inactivate the replicating DNA or RNA or viruses. UV-C photo-catalysis is particularly effective at removing bio-aerosols and other pollutants from indoor environments.

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